Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21

Expansion of NK cells from PBMCs using immobilized 4-1BBL and interleukin-21.

Adoptive transfer of NK cells has been widely applied clinically for cancer immunotherapy. However, the difficulties to obtain a large number of activated NK cells impede the successful application of such therapy. In the present study, we implemented a novel method involving the use of immobilized human 4-1BBL and interleukin-21 to amplify NK cells from the peripheral blood mononuclear cells (...

Interleukin-21 Enhances NK Cell Activation

Preferential T cell Expansion by Artificial Antigen Presenting Cells Expressing 4-1BBL Alone or in Combination with CD80 or CD86

The TNF receptor-ligands 4-1BB-4-1BBL and GITR-GITRL in NK cell responses

Interactions between several tumor necrosis factor (TNF)-TNF receptor (TNFR) superfamily members that are expressed by T cells and natural killer (NK) cells and various other cell types modulate immune responses. This review summarizes the current understanding of how the TNF ligand-TNFR interactions 4-1BBL with 4-1BB, and GITRL with glucocorticoid-induced TNFR-related (GITR) regulate NK cell m...

Culture and Expansion of Rat Mesenchymal Stem Cells Using the Serum Prepared From Rat’S Peripheral Blood

Purpose: The aim of this study is to evaluate the effects of prepared serum from rat peripheral blood on growth and viability of rat bone marrow-derived mesenchymal stem cells (MSCs) in vitro. Materials and Methods: Bone marrow cells prepared from rat’s tibia and femur central canal were cultured either in medium containing fetal bovin serum (FBS) or rat peripheral blood-derived serum (PBDS) f...